Xenopus laevis oocytes are a valuable tool for investigating the function of membrane
proteins. However, regulations around the world, specifically in Brazil, render the import of
Xenopus laevis frogs impractical, and, in some cases, impossible. Here, as an alternative,
we evaluate the usefulness of the North American aquatic bullfrog Lithobates
catesebeianus, which is commercially available in Brazil, for the heterologous expression of
aquaporin (AQP) proteins. We have developed a method that combines a brief collagenase …

ReP USP - Detalhe do registro: Lithobates catesbeianus (American Bullfrog) oocytes: a
novelheterologous expression system for aquaporins …